With this project an attempt is made to present and implement a proper methodology for the assessment of the optimal number of personnel for the efficient operation of the Cargo Station of Olympic Airways S.A. at the Athens International Airport. The optimal level of human resources is approached under 6 different scenarios concerning the degree of dedication of personnel and the level of demand for cargo transport. Conclusions are inferred about the preferred degree of specialisation of the labour force, the working hours and the allocation of personnel across the day. In parallel, issues concerning the influence of several other internal and external factors to the operation of the Cargo Station are also examined. The impact of all these elements implies that the estimation of the optimal number of personnel is an important, but not the sole solution for the improvement of the efficiency of the Cargo Station’s operation.